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Scanning confocal near-infra-red microscopy: a new microscopy technique for three-dimensional histopathology

Research output: Contribution to journalArticle

  • MSN Murthy
  • MG Jones
  • J Kulka
  • Jack D Davies
  • M Halliwell
  • PC Jackson
  • David Bull
  • PNT Wells
Original languageEnglish
Pages223 - 230
JournalEngineering Science and Education Journal
Journal publication dateOct 1995
Volume4
Journal issue5
DOIs
StatePublished
Peer-reviewedYes

Abstract

To image microstructures within tissue in detail using conventional light microscopy it is necessary to process the tissue and obtain thin (5 μm) physical sections by using destructive physical sectioning techniques. This article describes a microscopy technique, using near-infra-red light (700-1500 nm) as the source, which is able to image thin sections of thick-tissue specimens nondestructively. Images of contiguous two-dimensional thin sections obtained serially can be combined to generate a three-dimensional image of the structures being imaged.

Additional information

Publisher: Institution of Electrical Engineers (IEE)

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