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A Modular Vaccine Platform Combining Self‐Assembled Peptide Cages and Immunogenic Peptides

Research output: Contribution to journalArticle

Original languageEnglish
Article number1807357
Number of pages12
JournalAdvanced Functional Materials
Volume29
Issue number8
Early online date11 Jan 2019
DOIs
DateAccepted/In press - 19 Dec 2018
DateE-pub ahead of print - 11 Jan 2019
DatePublished (current) - 21 Feb 2019

Abstract

Subunit vaccines use delivery platforms to present minimal antigenic compo- nents for immunization. The benefits of such systems include multivalency, self-adjuvanting properties, and more specific immune responses. Previously, the design, synthesis, and characterization of self-assembling peptide cages (SAGEs) have been reported. In these, de novo peptides are combined to make hubs that assemble into nanoparticles when mixed in aqueous solu- tion. Here it is shown that SAGEs are nontoxic particles with potential as accessible synthetic peptide scaffolds for the delivery of immunogenic com- ponents. To this end, SAGEs functionalized with the model antigenic peptides tetanus toxoid632-651 and ovalbumin323-339 drive antigen-specific responses both in vitro and in vivo, eliciting both CD4+ T cell and B cell responses. Additionally, SAGEs functionalized with the antigenic peptide hemag- glutinin518-526 from the influenza virus are also able to drive a CD8+ T cell response in vivo. This work demonstrates the potential of SAGEs to act as
a modular scaffold for antigen delivery, capable of inducing and boosting specific and tailored immune responses.

    Research areas

  • coiled coils, peptide design, self-assembly, subunit vaccines, Synthetic biology

    Structured keywords

  • Bristol BioDesign Institute
  • BrisSynBio

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Documents

  • Full-text PDF (accepted author manuscript)

    Rights statement: This is the author accepted manuscript (AAM). The final published version (version of record) is available online via Wiley at https://onlinelibrary.wiley.com/doi/10.1002/adfm.201807357. Please refer to any applicable terms of use of the publisher.

    Accepted author manuscript, 3 MB, PDF document

    Embargo ends: 11/01/20

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