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An acid-compatible co-polymer for the solubilization of membranes and proteins into lipid bilayer-containing nanoparticles

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An acid-compatible co-polymer for the solubilization of membranes and proteins into lipid bilayer-containing nanoparticles. / Hall, Stephen C.L.; Tognoloni, Cecilia; Charlton, Jack; Bragginton, Éilís C.; Rothnie, Alice J.; Sridhar, Pooja; Wheatley, Mark; Knowles, Timothy J.; Arnold, Thomas; Edler, Karen J.; Dafforn, Tim R.

In: Nanoscale, Vol. 10, No. 22, 14.06.2018, p. 10609-10619.

Research output: Contribution to journalArticle

Harvard

Hall, SCL, Tognoloni, C, Charlton, J, Bragginton, ÉC, Rothnie, AJ, Sridhar, P, Wheatley, M, Knowles, TJ, Arnold, T, Edler, KJ & Dafforn, TR 2018, 'An acid-compatible co-polymer for the solubilization of membranes and proteins into lipid bilayer-containing nanoparticles' Nanoscale, vol. 10, no. 22, pp. 10609-10619. https://doi.org/10.1039/c8nr01322e

APA

Hall, S. C. L., Tognoloni, C., Charlton, J., Bragginton, É. C., Rothnie, A. J., Sridhar, P., ... Dafforn, T. R. (2018). An acid-compatible co-polymer for the solubilization of membranes and proteins into lipid bilayer-containing nanoparticles. Nanoscale, 10(22), 10609-10619. https://doi.org/10.1039/c8nr01322e

Vancouver

Author

Hall, Stephen C.L. ; Tognoloni, Cecilia ; Charlton, Jack ; Bragginton, Éilís C. ; Rothnie, Alice J. ; Sridhar, Pooja ; Wheatley, Mark ; Knowles, Timothy J. ; Arnold, Thomas ; Edler, Karen J. ; Dafforn, Tim R. / An acid-compatible co-polymer for the solubilization of membranes and proteins into lipid bilayer-containing nanoparticles. In: Nanoscale. 2018 ; Vol. 10, No. 22. pp. 10609-10619.

Bibtex

@article{b9d07f4c297146f2a6b84c45a0c7589d,
title = "An acid-compatible co-polymer for the solubilization of membranes and proteins into lipid bilayer-containing nanoparticles",
abstract = "The fundamental importance of membrane proteins in drug discovery has meant that membrane mimetic systems for studying membrane proteins are of increasing interest. One such system has been the amphipathic, negatively charged poly(styrene-co-maleic acid) (SMA) polymer to form {"}SMA Lipid Particles{"} (SMALPs) which have been widely adopted to solubilize membrane proteins directly from the cell membrane. However, SMALPs are only soluble under basic conditions and precipitate in the presence of divalent cations required for many downstream applications. Here, we show that the positively charged poly(styrene-co-maleimide) (SMI) forms similar nanoparticles with comparable efficiency to SMA, whilst remaining functional at acidic pH and compatible with high concentrations of divalent cations. We have performed a detailed characterization of the performance of SMI that enables a direct comparison with similar data published for SMA. We also demonstrate that SMI is capable of extracting proteins directly from the cell membrane and can solubilize functional human G-protein coupled receptors (GPCRs) expressed in cultured HEK 293T cells. {"}SMILPs{"} thus provide an alternative membrane solubilization method that successfully overcomes some of the limitations of the SMALP method.",
author = "Hall, {Stephen C.L.} and Cecilia Tognoloni and Jack Charlton and Bragginton, {{\'E}il{\'i}s C.} and Rothnie, {Alice J.} and Pooja Sridhar and Mark Wheatley and Knowles, {Timothy J.} and Thomas Arnold and Edler, {Karen J.} and Dafforn, {Tim R.}",
year = "2018",
month = "6",
day = "14",
doi = "10.1039/c8nr01322e",
language = "English",
volume = "10",
pages = "10609--10619",
journal = "Nanoscale",
issn = "2040-3364",
publisher = "Royal Society of Chemistry",
number = "22",

}

RIS - suitable for import to EndNote

TY - JOUR

T1 - An acid-compatible co-polymer for the solubilization of membranes and proteins into lipid bilayer-containing nanoparticles

AU - Hall, Stephen C.L.

AU - Tognoloni, Cecilia

AU - Charlton, Jack

AU - Bragginton, Éilís C.

AU - Rothnie, Alice J.

AU - Sridhar, Pooja

AU - Wheatley, Mark

AU - Knowles, Timothy J.

AU - Arnold, Thomas

AU - Edler, Karen J.

AU - Dafforn, Tim R.

PY - 2018/6/14

Y1 - 2018/6/14

N2 - The fundamental importance of membrane proteins in drug discovery has meant that membrane mimetic systems for studying membrane proteins are of increasing interest. One such system has been the amphipathic, negatively charged poly(styrene-co-maleic acid) (SMA) polymer to form "SMA Lipid Particles" (SMALPs) which have been widely adopted to solubilize membrane proteins directly from the cell membrane. However, SMALPs are only soluble under basic conditions and precipitate in the presence of divalent cations required for many downstream applications. Here, we show that the positively charged poly(styrene-co-maleimide) (SMI) forms similar nanoparticles with comparable efficiency to SMA, whilst remaining functional at acidic pH and compatible with high concentrations of divalent cations. We have performed a detailed characterization of the performance of SMI that enables a direct comparison with similar data published for SMA. We also demonstrate that SMI is capable of extracting proteins directly from the cell membrane and can solubilize functional human G-protein coupled receptors (GPCRs) expressed in cultured HEK 293T cells. "SMILPs" thus provide an alternative membrane solubilization method that successfully overcomes some of the limitations of the SMALP method.

AB - The fundamental importance of membrane proteins in drug discovery has meant that membrane mimetic systems for studying membrane proteins are of increasing interest. One such system has been the amphipathic, negatively charged poly(styrene-co-maleic acid) (SMA) polymer to form "SMA Lipid Particles" (SMALPs) which have been widely adopted to solubilize membrane proteins directly from the cell membrane. However, SMALPs are only soluble under basic conditions and precipitate in the presence of divalent cations required for many downstream applications. Here, we show that the positively charged poly(styrene-co-maleimide) (SMI) forms similar nanoparticles with comparable efficiency to SMA, whilst remaining functional at acidic pH and compatible with high concentrations of divalent cations. We have performed a detailed characterization of the performance of SMI that enables a direct comparison with similar data published for SMA. We also demonstrate that SMI is capable of extracting proteins directly from the cell membrane and can solubilize functional human G-protein coupled receptors (GPCRs) expressed in cultured HEK 293T cells. "SMILPs" thus provide an alternative membrane solubilization method that successfully overcomes some of the limitations of the SMALP method.

UR - http://www.scopus.com/inward/record.url?scp=85048281822&partnerID=8YFLogxK

U2 - 10.1039/c8nr01322e

DO - 10.1039/c8nr01322e

M3 - Article

VL - 10

SP - 10609

EP - 10619

JO - Nanoscale

T2 - Nanoscale

JF - Nanoscale

SN - 2040-3364

IS - 22

ER -