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An Esterase-like Lyase Catalyzes Acetate Elimination in Spirotetronate/Spirotetramate Biosynthesis

Research output: Contribution to journalArticle

Original languageEnglish
Pages (from-to)2305-2309
Number of pages5
JournalAngewandte Chemie - International Edition
Volume58
Issue number8
Early online date21 Jan 2019
DOIs
DateAccepted/In press - 20 Dec 2018
DateE-pub ahead of print - 21 Jan 2019
DatePublished (current) - 21 Jan 2019

Abstract

Spirotetronate and spirotetramate natural products include a multitude of compounds with potent antimicrobial and antitumor activities. Their biosynthesis incorporates many unusual biocatalytic steps, including regio- and stereo-specific modifications, cyclizations promoted by Diels–Alderases, and acetylation-elimination reactions. Here we focus on the acetate elimination catalyzed by AbyA5, implicated in the formation of the key Diels–Alder substrate to give the spirocyclic system of the antibiotic abyssomicin C. Using synthetic substrate analogues, it is shown that AbyA5 catalyzes stereospecific acetate elimination, establishing the (R)-tetronate acetate as a biosynthetic intermediate. The X-ray crystal structure of AbyA5, the first of an acetate-eliminating enzyme, reveals a deviant acetyl esterase fold. Molecular dynamics simulations and enzyme assays show the use of a His-Ser dyad to catalyze either elimination or hydrolysis, via disparate mechanisms, under substrate control.

    Research areas

  • antibiotics, biocatalysis, enzyme structure, enzymology, polyketides

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    Rights statement: This is the author accepted manuscript (AAM). The final published version (version of record) is available online via Wiley at https://onlinelibrary.wiley.com/doi/full/10.1002/anie.201812105 . Please refer to any applicable terms of use of the publisher.

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