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Characterisation of early and late bovine papillomavirus protein expression in equine sarcoids

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Characterisation of early and late bovine papillomavirus protein expression in equine sarcoids. / Wilson, A. D.; Armstrong, E. L. R.; Gofton, R. G.; Mason, Jenny; De Toit, N.; Day, M. J.

In: Veterinary Microbiology, Vol. 162, No. 2-4, 23.03.2013, p. 369-380.

Research output: Contribution to journalArticle

Harvard

Wilson, AD, Armstrong, ELR, Gofton, RG, Mason, J, De Toit, N & Day, MJ 2013, 'Characterisation of early and late bovine papillomavirus protein expression in equine sarcoids', Veterinary Microbiology, vol. 162, no. 2-4, pp. 369-380. https://doi.org/10.1016/j.vetmic.2012.10.010

APA

Wilson, A. D., Armstrong, E. L. R., Gofton, R. G., Mason, J., De Toit, N., & Day, M. J. (2013). Characterisation of early and late bovine papillomavirus protein expression in equine sarcoids. Veterinary Microbiology, 162(2-4), 369-380. https://doi.org/10.1016/j.vetmic.2012.10.010

Vancouver

Wilson AD, Armstrong ELR, Gofton RG, Mason J, De Toit N, Day MJ. Characterisation of early and late bovine papillomavirus protein expression in equine sarcoids. Veterinary Microbiology. 2013 Mar 23;162(2-4):369-380. https://doi.org/10.1016/j.vetmic.2012.10.010

Author

Wilson, A. D. ; Armstrong, E. L. R. ; Gofton, R. G. ; Mason, Jenny ; De Toit, N. ; Day, M. J. / Characterisation of early and late bovine papillomavirus protein expression in equine sarcoids. In: Veterinary Microbiology. 2013 ; Vol. 162, No. 2-4. pp. 369-380.

Bibtex

@article{aef82418204243a299b7fdc2a4bca41f,
title = "Characterisation of early and late bovine papillomavirus protein expression in equine sarcoids",
abstract = "Sarcoids are common skin tumours of horses and donkeys that are characterised by persistent proliferation of dermal fibroblasts associated with the presence of bovine papillomavirus (BPV) DNA. Some early BPV proteins have been demonstrated within sarcoids and RNA containing both early and late transcripts is present, yet it remains unclear whether late replication of BPV, culminating in the production of infectious virus particles, can occur in equids. Here we report that BPV1 RNA isolated from equine sarcoids encodes a unique deletion of four residues within the L2 protein suggesting a novel variant of virus has evolved in equines. Such viral evolution would require the production and transmission of virus particles among horses with sarcoids. Quantitative RT-PCR demonstrated the presence of mRNA transcripts containing early gene message in sarcoid tissues and BPV-E2 early virus antigen was detected by immunofluorescence in the nuclei of dermal fibroblasts, but no E2 expression could be detected within the overlying epidermis where productive virus replication would be expected to occur.Although immunohistochemistry clearly detected late virus proteins in the nuclei of dermal cells from samples of bovine papillomas, no late protein expression was detected in formalin-fixed tissue from equine sarcoids; either in the dermis or epidermis. Moreover, quantitative RT-PCR demonstrated that late gene mRNA represented",
keywords = "TUMORS, Equine, HORSES, E5, Tumour, CLINICAL TYPES, CELLS, SEQUENCE VARIANTS, TRANSFORMATION, TYPE-1, DNA, Papillomavirus, VIRUS, Sarcoid",
author = "Wilson, {A. D.} and Armstrong, {E. L. R.} and Gofton, {R. G.} and Jenny Mason and {De Toit}, N. and Day, {M. J.}",
year = "2013",
month = "3",
day = "23",
doi = "10.1016/j.vetmic.2012.10.010",
language = "English",
volume = "162",
pages = "369--380",
journal = "Veterinary Microbiology",
issn = "0378-1135",
publisher = "Elsevier",
number = "2-4",

}

RIS - suitable for import to EndNote

TY - JOUR

T1 - Characterisation of early and late bovine papillomavirus protein expression in equine sarcoids

AU - Wilson, A. D.

AU - Armstrong, E. L. R.

AU - Gofton, R. G.

AU - Mason, Jenny

AU - De Toit, N.

AU - Day, M. J.

PY - 2013/3/23

Y1 - 2013/3/23

N2 - Sarcoids are common skin tumours of horses and donkeys that are characterised by persistent proliferation of dermal fibroblasts associated with the presence of bovine papillomavirus (BPV) DNA. Some early BPV proteins have been demonstrated within sarcoids and RNA containing both early and late transcripts is present, yet it remains unclear whether late replication of BPV, culminating in the production of infectious virus particles, can occur in equids. Here we report that BPV1 RNA isolated from equine sarcoids encodes a unique deletion of four residues within the L2 protein suggesting a novel variant of virus has evolved in equines. Such viral evolution would require the production and transmission of virus particles among horses with sarcoids. Quantitative RT-PCR demonstrated the presence of mRNA transcripts containing early gene message in sarcoid tissues and BPV-E2 early virus antigen was detected by immunofluorescence in the nuclei of dermal fibroblasts, but no E2 expression could be detected within the overlying epidermis where productive virus replication would be expected to occur.Although immunohistochemistry clearly detected late virus proteins in the nuclei of dermal cells from samples of bovine papillomas, no late protein expression was detected in formalin-fixed tissue from equine sarcoids; either in the dermis or epidermis. Moreover, quantitative RT-PCR demonstrated that late gene mRNA represented

AB - Sarcoids are common skin tumours of horses and donkeys that are characterised by persistent proliferation of dermal fibroblasts associated with the presence of bovine papillomavirus (BPV) DNA. Some early BPV proteins have been demonstrated within sarcoids and RNA containing both early and late transcripts is present, yet it remains unclear whether late replication of BPV, culminating in the production of infectious virus particles, can occur in equids. Here we report that BPV1 RNA isolated from equine sarcoids encodes a unique deletion of four residues within the L2 protein suggesting a novel variant of virus has evolved in equines. Such viral evolution would require the production and transmission of virus particles among horses with sarcoids. Quantitative RT-PCR demonstrated the presence of mRNA transcripts containing early gene message in sarcoid tissues and BPV-E2 early virus antigen was detected by immunofluorescence in the nuclei of dermal fibroblasts, but no E2 expression could be detected within the overlying epidermis where productive virus replication would be expected to occur.Although immunohistochemistry clearly detected late virus proteins in the nuclei of dermal cells from samples of bovine papillomas, no late protein expression was detected in formalin-fixed tissue from equine sarcoids; either in the dermis or epidermis. Moreover, quantitative RT-PCR demonstrated that late gene mRNA represented

KW - TUMORS

KW - Equine

KW - HORSES

KW - E5

KW - Tumour

KW - CLINICAL TYPES

KW - CELLS

KW - SEQUENCE VARIANTS

KW - TRANSFORMATION

KW - TYPE-1

KW - DNA

KW - Papillomavirus

KW - VIRUS

KW - Sarcoid

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U2 - 10.1016/j.vetmic.2012.10.010

DO - 10.1016/j.vetmic.2012.10.010

M3 - Article

VL - 162

SP - 369

EP - 380

JO - Veterinary Microbiology

JF - Veterinary Microbiology

SN - 0378-1135

IS - 2-4

ER -