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Early Signaling in Primary T Cells Activated by Antigen Presenting Cells Is Associated with a Deep and Transient Lamellal Actin Network

Research output: Contribution to journalArticle

Original languageEnglish
Article numbere0133299
Number of pages22
JournalPLoS ONE
Volume10
Issue number8
Early online date25 Jun 2015
DOIs
DateE-pub ahead of print - 25 Jun 2015
DatePublished (current) - 3 Aug 2015

Abstract

Cellular signaling transduction critically depends on molecular interactions that are in turn governed by dynamic subcellular distributions of the signaling system components. Comprehensive insight into signal transduction requires an understanding of such distributions and cellular structures driving them. To investigate the activation of primary murine T cells by antigen presenting cells (APC) we have imaged more than 60 signaling intermediates during T cell stimulation with microscopy across resolution limits. A substantial number of signaling intermediates associated with a transient, wide, and actin-associated lamellum extending from an interdigitated T cell:APC interface several micrometers into the T cell, as characterized in detail here. By mapping the more than 60 signaling intermediates onto the spatiotemporal features of cell biological structures, the lamellum and other ones previously described, we also define distinct spatial and temporal characteristics of T cell signal initiation, amplification, and core signaling in the activation of primary T cells by APCs. These characteristics differ substantially from ones seen when T cells are activated using common reductionist approaches.

    Research areas

  • T cells, Actins, Cell signaling structures, TCR signaling cascade, Cell signaling, Antigen-Presenting cells, Fluorescence imaging, Cell membranes

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