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Functional consequences of co-expressing connexin40 or connexin45 with connexin43 on intercellular electrical coupling

Research output: Contribution to journalArticle

  • Neil M. Thomas
  • Rosaire Gray
  • Christopher H. Fry
  • Thomas Desplantez
  • Nicholas S. Peters
  • Nicholas J. Severs
  • Kenneth T. Macleod
  • Emmanuel Dupont
Original languageEnglish
Pages (from-to)191-196
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume483
Issue number1
Early online date26 Dec 2016
DOIs
DateAccepted/In press - 25 Dec 2016
DateE-pub ahead of print - 26 Dec 2016
DatePublished (current) - 29 Jan 2017

Abstract

The functional characteristics of the co-expression of connexin43, connexin40, and connexin45 proteins in human myocardium are thought to play an important role in governing normal propagation of the cardiac electrical impulse and in generating the myocardial substrate for some arrhythmias and conduction disturbances. A rat liver epithelial cell line, that endogenously expresses connexin43, was used to induce also expression of connexin40 or connexin45 after stable transfection using an inducible ecdysone system. Electrical coupling was estimated from measurement of the input resistance of transfected cells using an intracellular microelectrode to inject current and record changes to membrane potential. However, varied expression of the transfected connexin40 or connexin45 did not change electrical coupling, although connexin43/40 co-expression led to better coupling than connexin43/45 co-expression. Quantification of endogenous connexin43 expression, at both mRNA and protein levels, showed that it was altered in a manner dependent on the transfected connexin isotype. The data using rat liver epithelial cells indicate an increased electrical coupling upon expression of connexin40 and connexin43 but decreased coupling with connexin45 and connexin43 co-expression.

Additional information

25 December 2016

    Research areas

  • Connexin, Gap junction, Heart, Inducible expression, Rat liver epithelial cells

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