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Integration of light and circadian signals that regulate chloroplast transcription by a nuclear-encoded sigma factor

Research output: Contribution to journalArticle

Original languageEnglish
Pages (from-to)727–738
Number of pages12
JournalNew Phytologist
Volume213
Issue number2
Early online date15 Sep 2016
DOIs
DateAccepted/In press - 28 Jul 2016
DateE-pub ahead of print - 15 Sep 2016
DatePublished (current) - Jan 2017

Abstract

We investigated the signalling pathways that regulate chloroplast transcription in response to environmental signals. One mechanism controlling plastid transcription involves nuclear-encoded sigma subunits of plastid-encoded plastid RNA polymerase. Transcripts encoding the sigma factor SIG5 are regulated by light and the circadian clock. However, the extent to which a chloroplast target of SIG5 is regulated by light-induced changes in SIG5 expression is unknown. Moreover, the photoreceptor signalling pathways underlying the circadian regulation of chloroplast transcription by SIG5 are unidentified. We monitored the regulation of chloroplast transcription in photoreceptor and sigma factor mutants under controlled light regimes in Arabidopsis thaliana. We established that a chloroplast transcriptional response to light intensity was mediated by SIG5; a chloroplast transcriptional response to the relative proportions of red and far red light was regulated by SIG5 through phytochrome and photosynthetic signals; and the circadian regulation of chloroplast transcription by SIG5 was predominantly dependent on blue light and cryptochrome. Our experiments reveal the extensive integration of signals concerning the light environment by a single sigma factor to regulate chloroplast transcription. This may originate from an evolutionarily ancient mechanism that protects photosynthetic bacteria from high light stress, which subsequently became integrated with higher plant phototransduction networks.

    Research areas

  • Arabidopsis thaliana, Chloroplasts, Circadian rhythms, Photobiology, Signal transduction

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    Rights statement: This is the final published version of the article (version of record). It first appeared online via Wiley at http://onlinelibrary.wiley.com/doi/10.1111/nph.14176/abstract. Please refer to any applicable terms of use of the publisher.

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