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Integration of light and circadian signals that regulate chloroplast transcription by a nuclear-encoded sigma factor

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Integration of light and circadian signals that regulate chloroplast transcription by a nuclear-encoded sigma factor. / Belbin, Fiona E.; Noordally, Zeenat B.; Wetherill, Sarah J.; Atkins, Kelly A.; Franklin, Keara A.; Dodd, Antony N.

In: New Phytologist, Vol. 213, No. 2, 01.2017, p. 727–738.

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Belbin, Fiona E. ; Noordally, Zeenat B. ; Wetherill, Sarah J. ; Atkins, Kelly A. ; Franklin, Keara A. ; Dodd, Antony N. / Integration of light and circadian signals that regulate chloroplast transcription by a nuclear-encoded sigma factor. In: New Phytologist. 2017 ; Vol. 213, No. 2. pp. 727–738.

Bibtex

@article{2729390f76034b2a8729cebad15c5ff9,
title = "Integration of light and circadian signals that regulate chloroplast transcription by a nuclear-encoded sigma factor",
abstract = "We investigated the signalling pathways that regulate chloroplast transcription in response to environmental signals. One mechanism controlling plastid transcription involves nuclear-encoded sigma subunits of plastid-encoded plastid RNA polymerase. Transcripts encoding the sigma factor SIG5 are regulated by light and the circadian clock. However, the extent to which a chloroplast target of SIG5 is regulated by light-induced changes in SIG5 expression is unknown. Moreover, the photoreceptor signalling pathways underlying the circadian regulation of chloroplast transcription by SIG5 are unidentified. We monitored the regulation of chloroplast transcription in photoreceptor and sigma factor mutants under controlled light regimes in Arabidopsis thaliana. We established that a chloroplast transcriptional response to light intensity was mediated by SIG5; a chloroplast transcriptional response to the relative proportions of red and far red light was regulated by SIG5 through phytochrome and photosynthetic signals; and the circadian regulation of chloroplast transcription by SIG5 was predominantly dependent on blue light and cryptochrome. Our experiments reveal the extensive integration of signals concerning the light environment by a single sigma factor to regulate chloroplast transcription. This may originate from an evolutionarily ancient mechanism that protects photosynthetic bacteria from high light stress, which subsequently became integrated with higher plant phototransduction networks.",
keywords = "Arabidopsis thaliana, Chloroplasts, Circadian rhythms, Photobiology, Signal transduction",
author = "Belbin, {Fiona E.} and Noordally, {Zeenat B.} and Wetherill, {Sarah J.} and Atkins, {Kelly A.} and Franklin, {Keara A.} and Dodd, {Antony N.}",
year = "2017",
month = "1",
doi = "10.1111/nph.14176",
language = "English",
volume = "213",
pages = "727–738",
journal = "New Phytologist",
issn = "0028-646X",
publisher = "Wiley",
number = "2",

}

RIS - suitable for import to EndNote

TY - JOUR

T1 - Integration of light and circadian signals that regulate chloroplast transcription by a nuclear-encoded sigma factor

AU - Belbin, Fiona E.

AU - Noordally, Zeenat B.

AU - Wetherill, Sarah J.

AU - Atkins, Kelly A.

AU - Franklin, Keara A.

AU - Dodd, Antony N.

PY - 2017/1

Y1 - 2017/1

N2 - We investigated the signalling pathways that regulate chloroplast transcription in response to environmental signals. One mechanism controlling plastid transcription involves nuclear-encoded sigma subunits of plastid-encoded plastid RNA polymerase. Transcripts encoding the sigma factor SIG5 are regulated by light and the circadian clock. However, the extent to which a chloroplast target of SIG5 is regulated by light-induced changes in SIG5 expression is unknown. Moreover, the photoreceptor signalling pathways underlying the circadian regulation of chloroplast transcription by SIG5 are unidentified. We monitored the regulation of chloroplast transcription in photoreceptor and sigma factor mutants under controlled light regimes in Arabidopsis thaliana. We established that a chloroplast transcriptional response to light intensity was mediated by SIG5; a chloroplast transcriptional response to the relative proportions of red and far red light was regulated by SIG5 through phytochrome and photosynthetic signals; and the circadian regulation of chloroplast transcription by SIG5 was predominantly dependent on blue light and cryptochrome. Our experiments reveal the extensive integration of signals concerning the light environment by a single sigma factor to regulate chloroplast transcription. This may originate from an evolutionarily ancient mechanism that protects photosynthetic bacteria from high light stress, which subsequently became integrated with higher plant phototransduction networks.

AB - We investigated the signalling pathways that regulate chloroplast transcription in response to environmental signals. One mechanism controlling plastid transcription involves nuclear-encoded sigma subunits of plastid-encoded plastid RNA polymerase. Transcripts encoding the sigma factor SIG5 are regulated by light and the circadian clock. However, the extent to which a chloroplast target of SIG5 is regulated by light-induced changes in SIG5 expression is unknown. Moreover, the photoreceptor signalling pathways underlying the circadian regulation of chloroplast transcription by SIG5 are unidentified. We monitored the regulation of chloroplast transcription in photoreceptor and sigma factor mutants under controlled light regimes in Arabidopsis thaliana. We established that a chloroplast transcriptional response to light intensity was mediated by SIG5; a chloroplast transcriptional response to the relative proportions of red and far red light was regulated by SIG5 through phytochrome and photosynthetic signals; and the circadian regulation of chloroplast transcription by SIG5 was predominantly dependent on blue light and cryptochrome. Our experiments reveal the extensive integration of signals concerning the light environment by a single sigma factor to regulate chloroplast transcription. This may originate from an evolutionarily ancient mechanism that protects photosynthetic bacteria from high light stress, which subsequently became integrated with higher plant phototransduction networks.

KW - Arabidopsis thaliana

KW - Chloroplasts

KW - Circadian rhythms

KW - Photobiology

KW - Signal transduction

UR - http://www.scopus.com/inward/record.url?scp=84987733290&partnerID=8YFLogxK

U2 - 10.1111/nph.14176

DO - 10.1111/nph.14176

M3 - Article

VL - 213

SP - 727

EP - 738

JO - New Phytologist

JF - New Phytologist

SN - 0028-646X

IS - 2

ER -