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Interleukin-13 and interleukin-33 mRNA are underexpressed in the duodenal mucosa of German Shepherd dogs with chronic enteropathy

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Interleukin-13 and interleukin-33 mRNA are underexpressed in the duodenal mucosa of German Shepherd dogs with chronic enteropathy. / Kathrani, Aarti; Lezcano, Victor; Hall, Edward J; Jergens, Albert E; Seo, Yeon-Jung; Mochel, Jonathan P; Atherly, Todd; Allenspach, Karin.

In: Journal of Veterinary Internal Medicine, Vol. 33, No. 4, 01.07.2019, p. 1660-1668.

Research output: Contribution to journalArticle

Harvard

Kathrani, A, Lezcano, V, Hall, EJ, Jergens, AE, Seo, Y-J, Mochel, JP, Atherly, T & Allenspach, K 2019, 'Interleukin-13 and interleukin-33 mRNA are underexpressed in the duodenal mucosa of German Shepherd dogs with chronic enteropathy', Journal of Veterinary Internal Medicine, vol. 33, no. 4, pp. 1660-1668. https://doi.org/10.1111/jvim.15544

APA

Kathrani, A., Lezcano, V., Hall, E. J., Jergens, A. E., Seo, Y-J., Mochel, J. P., ... Allenspach, K. (2019). Interleukin-13 and interleukin-33 mRNA are underexpressed in the duodenal mucosa of German Shepherd dogs with chronic enteropathy. Journal of Veterinary Internal Medicine, 33(4), 1660-1668. https://doi.org/10.1111/jvim.15544

Vancouver

Kathrani A, Lezcano V, Hall EJ, Jergens AE, Seo Y-J, Mochel JP et al. Interleukin-13 and interleukin-33 mRNA are underexpressed in the duodenal mucosa of German Shepherd dogs with chronic enteropathy. Journal of Veterinary Internal Medicine. 2019 Jul 1;33(4):1660-1668. https://doi.org/10.1111/jvim.15544

Author

Kathrani, Aarti ; Lezcano, Victor ; Hall, Edward J ; Jergens, Albert E ; Seo, Yeon-Jung ; Mochel, Jonathan P ; Atherly, Todd ; Allenspach, Karin. / Interleukin-13 and interleukin-33 mRNA are underexpressed in the duodenal mucosa of German Shepherd dogs with chronic enteropathy. In: Journal of Veterinary Internal Medicine. 2019 ; Vol. 33, No. 4. pp. 1660-1668.

Bibtex

@article{51d578e663264b74b05aaaf0731505f6,
title = "Interleukin-13 and interleukin-33 mRNA are underexpressed in the duodenal mucosa of German Shepherd dogs with chronic enteropathy",
abstract = "Background: A recent genome-wide association study in German Shepherd dogs (GSDs) with chronic enteropathy (CE) has identified polymorphisms in the Th2 cytokine genes. Hypothesis/objective: To determine if the expression of the Th2 cytokines, interleukin-13 (IL-13) and interleukin-33 (IL-33), is altered in the duodenal mucosa of GSDs with CE compared to non-GSDs with CE and healthy dogs. Animals: Twenty client-owned dogs diagnosed with CE (10 GSDs and 10 non-GSDs) at the Bristol Veterinary School and 8 healthy Beagle dogs from the Iowa State University Service Colony. Methods: Retrospective study using archived paraffin-embedded duodenal biopsy samples. A novel RNA in situ hybridization technology (RNAscope) was used to hybridize IL-13 and IL-33 mRNA probes onto at least 10 sections from duodenal biopsy samples for each dog. RNAscope signals were visualized using a microscope and semi-quantitative assessment was performed by a single operator. Results: Based on duodenal villus, subvillus, epithelial, and lamina propria average expression scores, GSDs with CE had significantly lower IL-13 and IL-33 mRNA expression compared to non-GSDs with CE (IL-13, P <.04; IL-33, P <.02) and healthy Beagle dogs (IL-13, P <.02; IL-33, P <.004). Conclusions and Clinical Importance: Similar to human patients with ulcerative colitis, a subtype of human inflammatory bowel disease, these data indicate that Th2 cytokines may be involved in the pathogenesis of CE in GSDs.",
keywords = "bowel, canine, cytokine, duodenum, inflammatory, Th2",
author = "Aarti Kathrani and Victor Lezcano and Hall, {Edward J} and Jergens, {Albert E} and Yeon-Jung Seo and Mochel, {Jonathan P} and Todd Atherly and Karin Allenspach",
year = "2019",
month = "7",
day = "1",
doi = "10.1111/jvim.15544",
language = "English",
volume = "33",
pages = "1660--1668",
journal = "Journal of Veterinary Internal Medicine",
issn = "0891-6640",
publisher = "Wiley",
number = "4",

}

RIS - suitable for import to EndNote

TY - JOUR

T1 - Interleukin-13 and interleukin-33 mRNA are underexpressed in the duodenal mucosa of German Shepherd dogs with chronic enteropathy

AU - Kathrani, Aarti

AU - Lezcano, Victor

AU - Hall, Edward J

AU - Jergens, Albert E

AU - Seo, Yeon-Jung

AU - Mochel, Jonathan P

AU - Atherly, Todd

AU - Allenspach, Karin

PY - 2019/7/1

Y1 - 2019/7/1

N2 - Background: A recent genome-wide association study in German Shepherd dogs (GSDs) with chronic enteropathy (CE) has identified polymorphisms in the Th2 cytokine genes. Hypothesis/objective: To determine if the expression of the Th2 cytokines, interleukin-13 (IL-13) and interleukin-33 (IL-33), is altered in the duodenal mucosa of GSDs with CE compared to non-GSDs with CE and healthy dogs. Animals: Twenty client-owned dogs diagnosed with CE (10 GSDs and 10 non-GSDs) at the Bristol Veterinary School and 8 healthy Beagle dogs from the Iowa State University Service Colony. Methods: Retrospective study using archived paraffin-embedded duodenal biopsy samples. A novel RNA in situ hybridization technology (RNAscope) was used to hybridize IL-13 and IL-33 mRNA probes onto at least 10 sections from duodenal biopsy samples for each dog. RNAscope signals were visualized using a microscope and semi-quantitative assessment was performed by a single operator. Results: Based on duodenal villus, subvillus, epithelial, and lamina propria average expression scores, GSDs with CE had significantly lower IL-13 and IL-33 mRNA expression compared to non-GSDs with CE (IL-13, P <.04; IL-33, P <.02) and healthy Beagle dogs (IL-13, P <.02; IL-33, P <.004). Conclusions and Clinical Importance: Similar to human patients with ulcerative colitis, a subtype of human inflammatory bowel disease, these data indicate that Th2 cytokines may be involved in the pathogenesis of CE in GSDs.

AB - Background: A recent genome-wide association study in German Shepherd dogs (GSDs) with chronic enteropathy (CE) has identified polymorphisms in the Th2 cytokine genes. Hypothesis/objective: To determine if the expression of the Th2 cytokines, interleukin-13 (IL-13) and interleukin-33 (IL-33), is altered in the duodenal mucosa of GSDs with CE compared to non-GSDs with CE and healthy dogs. Animals: Twenty client-owned dogs diagnosed with CE (10 GSDs and 10 non-GSDs) at the Bristol Veterinary School and 8 healthy Beagle dogs from the Iowa State University Service Colony. Methods: Retrospective study using archived paraffin-embedded duodenal biopsy samples. A novel RNA in situ hybridization technology (RNAscope) was used to hybridize IL-13 and IL-33 mRNA probes onto at least 10 sections from duodenal biopsy samples for each dog. RNAscope signals were visualized using a microscope and semi-quantitative assessment was performed by a single operator. Results: Based on duodenal villus, subvillus, epithelial, and lamina propria average expression scores, GSDs with CE had significantly lower IL-13 and IL-33 mRNA expression compared to non-GSDs with CE (IL-13, P <.04; IL-33, P <.02) and healthy Beagle dogs (IL-13, P <.02; IL-33, P <.004). Conclusions and Clinical Importance: Similar to human patients with ulcerative colitis, a subtype of human inflammatory bowel disease, these data indicate that Th2 cytokines may be involved in the pathogenesis of CE in GSDs.

KW - bowel

KW - canine

KW - cytokine

KW - duodenum

KW - inflammatory

KW - Th2

UR - http://www.scopus.com/inward/record.url?scp=85067665941&partnerID=8YFLogxK

U2 - 10.1111/jvim.15544

DO - 10.1111/jvim.15544

M3 - Article

VL - 33

SP - 1660

EP - 1668

JO - Journal of Veterinary Internal Medicine

JF - Journal of Veterinary Internal Medicine

SN - 0891-6640

IS - 4

ER -