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Investigating the Origins of Membrane Phospholipid Biosynthesis Genes Using Outgroup-Free Rooting

Research output: Contribution to journalArticle

Original languageEnglish
Article numberevz034
Pages (from-to)883-898
Number of pages16
JournalGenome Biology and Evolution
Volume11
Issue number3
Early online date8 Feb 2019
DOIs
DateAccepted/In press - 30 Jan 2019
DateE-pub ahead of print - 8 Feb 2019
DatePublished (current) - 1 Mar 2019

Abstract

One of the key differences between Bacteria and Archaea is their canonical membrane phospholipids, which are synthesized by distinct biosynthetic pathways with nonhomologous enzymes. This "lipid divide" has important implications for the early evolution of cells and the type of membrane phospholipids present in the last universal common ancestor. One of the main challenges in studies of membrane evolution is that the key biosynthetic genes are ancient and their evolutionary histories are poorly resolved. This poses major challenges for traditional rooting methods because the only available outgroups are distantly related. Here, we address this issue by using the best available substitution models for single-gene trees, by expanding our analyses to the diversity of uncultivated prokaryotes recently revealed by environmental genomics, and by using two complementary approaches to rooting that do not depend on outgroups. Consistent with some previous analyses, our rooted gene trees support extensive interdomain horizontal transfer of membrane phospholipid biosynthetic genes, primarily from Archaea to Bacteria. They also suggest that the capacity to make archaeal-type membrane phospholipids was already present in last universal common ancestor.

    Research areas

  • lipid divide, lipid evolution, outgroup-free rooting, phylogenetics

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    Rights statement: This is the final published version of the article (version of record). It first appeared online via Oxford University Press at https://academic.oup.com/gbe/article/11/3/883/5310093. Please refer to any applicable terms of use of the publisher.

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