Skip to content

Mast cells in early rheumatoid arthritis associate with disease severity and support B-cell autoantibody production

Research output: Contribution to journalArticle

  • Felice Rivellese
  • Daniele Mauro
  • Alessandra Nerviani
  • Sara Pagani
  • Liliane Fossati-Jimack
  • Tobias Messemaker
  • Fina Kurreeman
  • Rene Toes
  • Simon Rauber
  • Georg Schett
  • Gareth Jones
  • Simon A. Jones
  • Francesca Rossi
  • Amato de Paulis
  • Gianni Marone
  • Mohey Eldin El Shikh
  • Frances Humby
  • Constantino Pitzalis
Original languageEnglish
Pages (from-to)1773-1781
Number of pages9
JournalAnnals of the Rheumatic Diseases
Issue number12
Early online date20 Aug 2018
DateAccepted/In press - 27 Jul 2018
DateE-pub ahead of print - 20 Aug 2018
DatePublished (current) - 1 Dec 2018


Objectives Mast cells (MCs) are involved in the pathogenesis of rheumatoid arthritis (RA). However, their contribution remains controversial. To establish their role in RA, we analysed their presence in the synovium of treatment-naïve patients with early RA and their association and functional relationship with histological features of synovitis. Methods Synovial tissue was obtained by ultrasound-guided biopsy from treatment-naïve patients with early RA (n=99). Immune cells (CD3/CD20/CD138/CD68) and their relationship with CD117+MCs in synovial tissue were analysed by immunohistochemistry (IHC) and immunofluorescence (IF). The functional involvement of MCs in ectopic lymphoid structures (ELS) was investigated in vitro, by coculturing MCs with naïve B cells and anticitrullinated protein antibodies (ACPA)-producing B cell clones, and in vivo in interleukin-27 receptor alpha (IL27ra)-deficient and control mice during antigen-induced arthritis (AIA). Results High synovial MC counts are associated with local and systemic inflammation, autoantibody positivity and high disease activity. IHC/IF showed that MCs reside at the outer border of lymphoid aggregates. Furthermore, human MCs promote the activation and differentiation of naïve B cells and induce the production of ACPA, mainly via contact-dependent interactions. In AIA, synovial MC numbers increase in IL27ra deficient mice, in association with ELS and worse disease activity.

Download statistics

No data available



  • Full-text PDF (accepted author manuscript)

    Rights statement: This is the author accepted manuscript (AAM). The final published version (version of record) is available online via BMJ at Please refer to any applicable terms of use of the publisher.

    Accepted author manuscript, 1 MB, PDF document

    Licence: CC BY-NC



View research connections

Related faculties, schools or groups