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Microbial use of low molecular weight DOM in filtered and unfiltered freshwater: Role of ultra-small microorganisms and implications for water quality monitoring

Research output: Contribution to journalArticle

Original languageEnglish
Pages (from-to)377-384
Number of pages8
JournalScience of The Total Environment
Volume598
Early online date25 Apr 2017
DOIs
DateAccepted/In press - 7 Apr 2017
DateE-pub ahead of print - 25 Apr 2017
DatePublished (current) - 15 Nov 2017

Abstract

Dissolved organic matter (DOM) plays a central role in regulating productivity and nutrient cycling in freshwaters. It is therefore vital that we can representatively sample and preserve DOM in freshwaters for subsequent analysis. Here we investigated the effect of filtration, temperature (5 and 25 °C) and acidification (HCl) on the persistence of low molecular weight (MW) dissolved organic carbon (DOC), nitrogen (DON) and orthophosphate in oligotrophic and eutrophic freshwater environments. Our results showed the rapid loss of isotopically-labelled glucose and amino acids from both filtered (0.22 and 0.45 μm) and unfiltered waters. We ascribe this substrate depletion in filtered samples to the activity of ultra-small (< 0.45 μm) microorganisms (bacteria and archaea) present in the water. As expected, the rate of C, N and P loss was much greater at higher temperatures and was repressed by the addition of HCl. Based on our results and an evaluation of the protocols used in recently published studies, we conclude that current techniques used to sample water for low MW DOM characterisation are frequently inadequate and lack proper validation. In contrast to the high degree of analytical precision and rigorous statistical analysis of most studies, we argue that insufficient consideration is still given to the presence of ultra-small microorganisms and potential changes that can occur in the low MW fraction of DOM prior to analysis.

    Research areas

  • Biodegradation, Metabolomics, Sampling method, Nutrients, Ultramicrobacteria, Uptake kinetics

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    Rights statement: This is the author accepted manuscript (AAM). The final published version (version of record) is available online via Elsevier at http://www.sciencedirect.com/science/article/pii/S0048969717308847. Please refer to any applicable terms of use of the publisher

    Accepted author manuscript, 835 KB, PDF document

    Licence: CC BY-NC-ND

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