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Modest Interference with Actin Dynamics in Primary T Cell Activation by Antigen Presenting Cells Preferentially Affects Lamellal Signaling

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Modest Interference with Actin Dynamics in Primary T Cell Activation by Antigen Presenting Cells Preferentially Affects Lamellal Signaling. / Roybal, Kole T; Mace, Emily M; Clark, Danielle J; Leard, Alan D; Herman, Andrew; Verkade, Paul; Orange, Jordan S; Wülfing, Christoph.

In: PLoS ONE, Vol. 10, No. 8, 03.08.2015, p. e0133231.

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Roybal, Kole T ; Mace, Emily M ; Clark, Danielle J ; Leard, Alan D ; Herman, Andrew ; Verkade, Paul ; Orange, Jordan S ; Wülfing, Christoph. / Modest Interference with Actin Dynamics in Primary T Cell Activation by Antigen Presenting Cells Preferentially Affects Lamellal Signaling. In: PLoS ONE. 2015 ; Vol. 10, No. 8. pp. e0133231.

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@article{dda0c97ced5c46e7a1f7003dda419996,
title = "Modest Interference with Actin Dynamics in Primary T Cell Activation by Antigen Presenting Cells Preferentially Affects Lamellal Signaling",
abstract = "Dynamic subcellular distributions of signaling system components are critical regulators of cellular signal transduction through their control of molecular interactions. Understanding how signaling activity depends on such distributions and the cellular structures driving them is required for comprehensive insight into signal transduction. In the activation of primary murine T cells by antigen presenting cells (APC) signaling intermediates associate with various subcellular structures, prominently a transient, wide, and actin-associated lamellum extending from an interdigitated T cell:APC interface several micrometers into the T cell. While actin dynamics are well established as general regulators of cellular organization, their role in controlling signaling organization in primary T cell:APC couples and the specific cellular structures driving it is unresolved. Using modest interference with actin dynamics with a low concentration of Jasplakinolide as corroborated by costimulation blockade we show that T cell actin preferentially controls lamellal signaling localization and activity leading downstream to calcium signaling. Lamellal localization repeatedly related to efficient T cell function. This suggests that the transient lamellal actin matrix regulates T cell signaling associations that facilitate T cell activation.",
keywords = "T cells, Actins, Calcium signaling, Antigen-Presenting cells, Cell Signaling, Cell membranes, Fluorescence recovery after photobleaching, Phosphorylation",
author = "Roybal, {Kole T} and Mace, {Emily M} and Clark, {Danielle J} and Leard, {Alan D} and Andrew Herman and Paul Verkade and Orange, {Jordan S} and Christoph W{\"u}lfing",
year = "2015",
month = "8",
day = "3",
doi = "10.1371/journal.pone.0133231",
language = "English",
volume = "10",
pages = "e0133231",
journal = "PLoS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "8",

}

RIS - suitable for import to EndNote

TY - JOUR

T1 - Modest Interference with Actin Dynamics in Primary T Cell Activation by Antigen Presenting Cells Preferentially Affects Lamellal Signaling

AU - Roybal, Kole T

AU - Mace, Emily M

AU - Clark, Danielle J

AU - Leard, Alan D

AU - Herman, Andrew

AU - Verkade, Paul

AU - Orange, Jordan S

AU - Wülfing, Christoph

PY - 2015/8/3

Y1 - 2015/8/3

N2 - Dynamic subcellular distributions of signaling system components are critical regulators of cellular signal transduction through their control of molecular interactions. Understanding how signaling activity depends on such distributions and the cellular structures driving them is required for comprehensive insight into signal transduction. In the activation of primary murine T cells by antigen presenting cells (APC) signaling intermediates associate with various subcellular structures, prominently a transient, wide, and actin-associated lamellum extending from an interdigitated T cell:APC interface several micrometers into the T cell. While actin dynamics are well established as general regulators of cellular organization, their role in controlling signaling organization in primary T cell:APC couples and the specific cellular structures driving it is unresolved. Using modest interference with actin dynamics with a low concentration of Jasplakinolide as corroborated by costimulation blockade we show that T cell actin preferentially controls lamellal signaling localization and activity leading downstream to calcium signaling. Lamellal localization repeatedly related to efficient T cell function. This suggests that the transient lamellal actin matrix regulates T cell signaling associations that facilitate T cell activation.

AB - Dynamic subcellular distributions of signaling system components are critical regulators of cellular signal transduction through their control of molecular interactions. Understanding how signaling activity depends on such distributions and the cellular structures driving them is required for comprehensive insight into signal transduction. In the activation of primary murine T cells by antigen presenting cells (APC) signaling intermediates associate with various subcellular structures, prominently a transient, wide, and actin-associated lamellum extending from an interdigitated T cell:APC interface several micrometers into the T cell. While actin dynamics are well established as general regulators of cellular organization, their role in controlling signaling organization in primary T cell:APC couples and the specific cellular structures driving it is unresolved. Using modest interference with actin dynamics with a low concentration of Jasplakinolide as corroborated by costimulation blockade we show that T cell actin preferentially controls lamellal signaling localization and activity leading downstream to calcium signaling. Lamellal localization repeatedly related to efficient T cell function. This suggests that the transient lamellal actin matrix regulates T cell signaling associations that facilitate T cell activation.

KW - T cells

KW - Actins

KW - Calcium signaling

KW - Antigen-Presenting cells

KW - Cell Signaling

KW - Cell membranes

KW - Fluorescence recovery after photobleaching

KW - Phosphorylation

U2 - 10.1371/journal.pone.0133231

DO - 10.1371/journal.pone.0133231

M3 - Article

VL - 10

SP - e0133231

JO - PLoS ONE

T2 - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 8

ER -