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Spatial Positioning and Chemical Coupling in Coacervate-in-Proteinosome Protocells

Research output: Contribution to journalArticle

Original languageEnglish
Number of pages6
JournalAngewandte Chemie - International Edition
Early online date22 May 2019
DOIs
DateAccepted/In press - 25 Apr 2019
DateE-pub ahead of print - 22 May 2019
DatePublished (current) - 1 Jul 2019

Abstract

The integration of molecularly crowded micro-environments into membrane-enclosed protocell models represents a step towards more realistic representations of cellular structure and organization. Herein, the membrane diffusion-mediated nucleation of negatively or positively charged coacervate micro-droplets within the aqueous lumen of individual proteinosomes is used to prepare nested hybrid protocells with spatially organized and chemically coupled enzyme activities. The location and reconfiguration of the entrapped droplets are regulated by tuning the electrostatic interactions between the encapsulated coacervate and surrounding negatively charged proteinosome membrane. As a consequence, alternative modes of a cascade reaction involving membrane- and coacervate-segregated enzymes can be implemented within the coacervate-in-proteinosome protocells.

    Research areas

  • coacervates, enzymes, membranes, proteinosomes, self-assembly

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    Rights statement: This is the final published version of the article (version of record). It first appeared online via Wiley at https://doi.org/10.1002/anie.201903756 . Please refer to any applicable terms of use of the publisher.

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