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Studying the subcellular localization and DNA-binding activity of FoxO transcription factors, downstream effectors of PI3K/Akt

Research output: Contribution to journalArticle

  • Abdelkader Essafi
  • Ana R Gomes
  • Karen M Pomeranz
  • Aleksandra K Zwolinska
  • Rana Varshochi
  • Ursula B McGovern
  • Eric W-F Lam
Original languageEnglish
Pages (from-to)201-11
Number of pages11
JournalMethods in Molecular Biology
DatePublished - 2009


This chapter describes methods for studying downstream events of the PI3K/Akt signaling cascade, focusing on the FoxO transcription factors. These approaches also represent alternative means for gauging the phosphoinositide-3 kinase/Akt activity. We describe protocols for the fractionation of cytoplasmic and nuclear protein extracts and for studying transcription factor DNA-binding activity in vitro and in vivo.

    Research areas

  • Base Sequence, Cell Line, Tumor, Cell Nucleus, Chemical Precipitation, Chromatin Immunoprecipitation, Cytosol, DNA, Forkhead Transcription Factors, Humans, Intracellular Space, Nuclear Proteins, Phosphatidylinositol 3-Kinases, Polymerase Chain Reaction, Protein Transport, Proto-Oncogene Proteins c-akt, Signal Transduction, Sonication, Journal Article


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