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Systems Imaging of the Immune Synapse

Research output: Chapter in Book/Report/Conference proceedingChapter in a book

Original languageEnglish
Title of host publicationThe Immune Synapse
Subtitle of host publicationMethods and Protocols
Publisher or commissioning bodyHumana Press
ISBN (Electronic)978-1-4939-6881-7
ISBN (Print)978-1-4939-6879-4
DateE-pub ahead of print - 3 Mar 2017
DatePublished (current) - 2017

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


Three‐dimensional live cell imaging of the interaction of T cells with antigen presenting cells (APC) visualises the subcellular distributions of signalling intermediates during T cell activation at thousands of resolved positions within a cell. These information-rich maps of local protein concentrations are a valuable resource in understanding T cell signalling. Here, we describe a protocol for the efficient acquisition of such imaging data and their computational processing to create four-dimensional maps of local concentrations. This protocol allows quantitative analysis of T cell signalling as it occurs inside live cells with resolution in time and space across thousands of cells.

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