Skip to content

Systems Imaging of the Immune Synapse

Research output: Chapter in Book/Report/Conference proceedingChapter in a book

Original languageEnglish
Title of host publicationThe Immune Synapse
Subtitle of host publicationMethods and Protocols
Publisher or commissioning bodyHumana Press
Pages409-423
Volume1584
ISBN (Electronic)978-1-4939-6881-7
ISBN (Print)978-1-4939-6879-4
DOIs
DateE-pub ahead of print - 3 Mar 2017
DatePublished (current) - 2017

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Abstract

Three‐dimensional live cell imaging of the interaction of T cells with antigen presenting cells (APC) visualises the subcellular distributions of signalling intermediates during T cell activation at thousands of resolved positions within a cell. These information-rich maps of local protein concentrations are a valuable resource in understanding T cell signalling. Here, we describe a protocol for the efficient acquisition of such imaging data and their computational processing to create four-dimensional maps of local concentrations. This protocol allows quantitative analysis of T cell signalling as it occurs inside live cells with resolution in time and space across thousands of cells.

Download statistics

No data available

Documents

Documents

DOI

View research connections

Related faculties, schools or groups