Skip to content

Zebrafish sp7 mutants show tooth cycling independent of attachment, eruption and poor differentiation of teeth

Research output: Contribution to journalArticle

Original languageEnglish
Pages (from-to)176-184
Number of pages9
JournalDevelopmental Biology
Volume435
Issue number2
Early online date2 Feb 2018
DOIs
DateAccepted/In press - 30 Jan 2018
DateE-pub ahead of print - 2 Feb 2018
DatePublished (current) - 15 Mar 2018

Abstract

The capacity to fully replace teeth continuously makes zebrafish an attractive model to explore regeneration and tooth development. The requirement of attachment bone for the appearance of replacement teeth has been hypothesized but not yet investigated. The transcription factor sp7 (osterix) is known in mammals to play an important role during odontoblast differentiation and root formation. Here we study tooth replacement in the absence of attachment bone using sp7 zebrafish mutants. We analysed the pattern of tooth replacement at different stages of development and demonstrated that in zebrafish lacking sp7, attachment bone is never present, independent of the stage of tooth development or fish age, yet replacement is not interrupted. Without bone of attachment we observed abnormal orientation of teeth, and abnormal connection of pulp cavities of predecessor and replacement teeth. Mutants lacking sp7 show arrested dentinogenesis, with non-polarization of odontoblasts and only a thin layer of dentin deposited. Osteoclast activity was observed in sp7 mutants; due to the lack of bone of attachment, remodelling was diminished but nevertheless present along the pharyngeal bone. We conclude that tooth replacement is ongoing in the sp7 mutant despite poor differentiation and defective attachment. Without bone of attachment tooth orientation and pulp organization are compromised.

Download statistics

No data available

Documents

Documents

  • Full-text PDF (accepted author manuscript)

    Rights statement: This is the author accepted manuscript (AAM). The final published version (version of record) is available online via Elsevier at https://www.sciencedirect.com/science/article/pii/S0012160617307455?via%3Dihub. Please refer to any applicable terms of use of the publisher.

    Accepted author manuscript, 288 KB, PDF document

  • Figures 1-5 PDF

    Accepted author manuscript, 8 MB, PDF document

DOI

View research connections

Related faculties, schools or groups